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In contrast to oligo(dT) primers, these primers are certainly not template particular and are ideal for DNA synthesis employing Klenow fragments with DNA templates or for cDNA synthesis applying reverse transcriptase with mRNA templates.
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Help Small complexity areas are some locations inside a DNA sequence which have biased foundation compositions such as a extend of ACACACACACACACACACA. Inner hybridization oligo parameters
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Assistance This controls whether the primer must span an exon junction on the mRNA template. The option "Primer will have to span an exon-exon junction" will immediate the program to return at the least a person primer (inside a supplied primer pair) that spans an exon-exon junction.
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